descargar manual de maquinas herramientas kibbe

International Nuclear Information System (INIS) Remotely adjustable fishing jar and method for using same Growth of a variety of anaerobic oral bacteria was comparable to that obtained after anaerobic incubation of broth cultures in Brewer Anaerobic Jars. A special disposable rubber flask closure maintains anaerobic conditions in the flask after autoclaving. Cultures can be grown in commercially available flasks normally used for preparation of sterile external solutions. Simple and convenient method for culturing anaerobic bacteria.īehbehani, M J Jordan, H V Santoro, D LĪ simple and convenient method for culturing anaerobic bacteria is described. This modified technique needs to be tested for many other clinical materials which are not yet evaluated. Interpretation & conclusions: For isolation of anaerobes from clinical specimens such as diabetic foot ulcers, modified candle jar technique was found to be as reliable as GasPak system. The most sensitive drug was piperacillin-tazobactam. Aerobic GNB were found to be multidrug resistant, especially to penicillin and cephalosporins. Imipenem and metronidazole were most sensitive while clindamycin, penicillin and cefoxitin were least sensitive drugs for anaerobes. and Clostridium perfringens by both GasPak and in-house developed and modified candle jar techniques. Anaerobes isolated from 21 samples were Peptostreptococcus, Bacteroides, Porphyromonas, Veillonella spp. Results: All the 43 samples were culture positive, of which aerobic Gram-negative bacteria (GNB predominated, followed by Staphylococcus aureus, Enterococcus and diphtheroids. Isolation of bacteria from diabetic foot ulcers with special reference to anaerobe isolation by simple two-step combustion technique in candle jarĭirectory of Open Access Journals (Sweden) Evidently, the Anoxomat method is more reliable and appears to support the growth of strict anaerobes better. The Anoxomat system provided superior growth, in terms of density and colony size, and achieved anaerobiosis more rapidly. The zones of inhibition of the antibiotics tested were not different in the two systems. The density of growth recorded for 177 (78%) strains was heavier in the Anoxomat than in the GasPak jar. With the Anoxomat, anaerobiosis was achieved about 35 min faster than in the GasPak system. The Porphyromonas asaccharolytica strains failed to grow within the GasPak system but grew inside the Anoxomat. There was no measurable difference in the colony sizes of the reference strains. The remaining 12% were equal in their sizes.

Of the 227 strains tested, the colonies of 152 (67%) were larger (by size range of 0.2-2.4 mm) in the Anoxomat at 48 h than in the GasPak jar compared with only 21% (range 0.1-0.3 mm) that were larger in the GasPak than in the Anoxomat. The results demonstrate that the growth of anaerobic bacteria is faster inside the Anoxomat jar than in the anaerobic GasPak jar system. A total of 227 obligate anaerobic bacteria comprising 116 stock strains, 5 ATCC reference strains and 106 fresh strains, representing different genera, were investigated for growth on anaerobic agar plates and scored for density, colony sizes, susceptibility zones of antibiotic inhibition and the speed of anaerobiosis (reducing the indicator). An anaerobic indicator within the jars monitored anaerobiosis. Anoxomat system utilized a gas mixture of 80% N(2), 10% CO(2) and 10% H(2), while the GasPak used a gas mixture of 90% H(2) and 10% CO(2). Anoxomat, model WS800, and anaerobic GasPak jar system (Oxoid) were evaluated. To evaluate the performance of the Anoxomat, in comparison with the conventional anaerobic GasPak jar system, for the isolation of obligate anaerobes. Shahin, May Jamal, Wafaa Verghese, Tina Rotimi, V O Comparative evaluation of anoxomat and conventional anaerobic GasPak jar systems for the isolation of anaerobic bacteria.

Descargar manual de maquinas herramientas kibbe